Name: Recombinant Mouse Carbonic Anhydrase IX / CA9 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0558
Availability: InStock

Recombinant Mouse Carbonic Anhydrase IX / CA9 Protein (His Tag)

Catalog No.: BLPSN-0558

Product Overview

Tag	His
Host Species	Mouse
Accession	NP_647466.2
Background	Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding the extracellular domain of mouse CAR9 (NP_647466.2) (Met 1-Asp 390) was expressed, with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Gln 32
AA Sequence	Met 1-Asp 390
Molecular Weight	The secreted recombinant mouse CAR9 comprises 370 a.a. and has a calculated molecular mass of 40.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its esterase activity. The specific activity is >100 pmoles/min/ug.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.