Recombinant Mouse Carbonic Anhydrase IV / CA4 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0553
Recombinant Mouse Carbonic Anhydrase IV / CA4 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0553
Catalog No.: BLPSN-0553
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | NP_031633.1 |
| Synonym | AW456718, Ca4, Car4, RP23-167D6.1 |
| Background | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 15-fold compared to CAII. |
| Description | A DNA sequence encoding the mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed,with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Glu 18 |
| AA Sequence | Met 1-Ser 277 |
| Molecular Weight | The secreted recombinant mouse Car4 consists of 271 a.a. and has a calculated molecular mass of 31 kDa as estimated in SDS-PAGE under reducing conditions. |
| Purity | >98% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its esterase activity. The specific activity is >10 pmoles/min/ug. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
