Recombinant Mouse Carbonic anhydrase II / CA2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0549
Recombinant Mouse Carbonic anhydrase II / CA2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0549
Catalog No.: BLPSN-0549
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | AAH55291.1 |
| Synonym | AI131712, Ca2, CAII, Car-2, Ltw-5, Lvtw-5 |
| Background | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human alpha carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1. |
| Description | A DNA sequence encoding the mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal His tag. |
| Source | E.coli |
| Predicted N Terminal | Ser 2 |
| AA Sequence | Met 1-Lys 260 |
| Molecular Weight | The recombinant mouse CA2 comprises 270 a.a. and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >96% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Measured by its esterase activity. The specific activity is >100 pmoles/min/ug. |
| Formulation | Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
