Recombinant Mouse B2M Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0392

Recombinant Mouse B2M Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0392
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Product Overview

Tag His
Host Species Mouse
Accession P01887
Synonym beta2-m, beta2m, Ly-m11
Background B2M, also known as beta2-Microglobulin or CDABP92, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(beta2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(beta2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(beta2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to thealpha3 chain. Unlikealpha3, beta2 has no transmembrane region. Directly above beta2 lies the alpha1 chain, which itself is lateral to the alpha2. In the absence of B2M(beta2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(beta2 microglobulin) can indicate non-progression of HIV.
Description A DNA sequence encoding the mouse B2M (P01887) (Met 1-Met 119), without the propeptide, was fused with a C-terminal His tag.
Source HEK293
Predicted N Terminal Ile 21
AA Sequence Met 1-Met 119
Molecular Weight The secreted recombinant mouse B2M comprises 110 a.a. and has a calculated molecular mass of 13 kDa. The apparent molecular mass of rmB2M is approximately 15 kDa in SDS-PAGE under reducing conditions.
Purity >97% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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