Name: Recombinant Mouse ASAM Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0276
Availability: InStock

Recombinant Mouse ASAM Protein (His Tag)

Catalog No.: BLPSN-0276

Product Overview

Tag	His
Host Species	Mouse
Accession	Q8R373
Synonym	9030425E11Rik, ACAM, ASP5, AW557819
Background	Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.
Description	A DNA sequence encoding the extracellular domain of mouse ASAM (Q8R373-1) (Met 1-Met 232) was expressed, with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Thr 18
AA Sequence	Met 1-Met 232
Molecular Weight	The recombinant mouse ASAM consists of 226 a.a. and has a predicted molecular mass of 25.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ASAM is approximately 33-36 kDa due to glycosylation.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 10E4 cells/well are added to mouse ASAM coated plates (30 ug/ml, 100 ul/well), approximately >40 % will adhere after one hour at 37 °C.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.