Name: Recombinant Mouse ASAH2 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0274
Availability: InStock

Recombinant Mouse ASAH2 Protein (His Tag)

Catalog No.: BLPSN-0274

Product Overview

Tag	His
Host Species	Mouse
Accession	NP_061300.1
Synonym	AI585898
Background	ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses -€˜amucin box-€™, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
Description	A DNA sequence encoding the Lumenal domain (Thr 34-Thr 756) of mouse ASAH2 (NP_061300.1) precursor was expressed with a N-terminal His tag.
Source	HEK293
Predicted N Terminal	His
AA Sequence	Thr 34-Thr 756
Molecular Weight	The secreted recombinant mouse ASAH2 consists of 739 a.a. and has a calculated molecular mass of 82 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 105-115 kDa protein in SDS-PAGE under reducing conditions.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid.The specific activity is > 3,000 pmoles/min/ug.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.