Recombinant Mouse ASAH2 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0274
Recombinant Mouse ASAH2 Protein (His Tag)

Recombinant Mouse ASAH2 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0274
Catalog No.: BLPSN-0274

Product Overview

Tag His
Host Species Mouse
Accession NP_061300.1
Synonym AI585898
Background ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses -€˜amucin box-€™, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
Description A DNA sequence encoding the Lumenal domain (Thr 34-Thr 756) of mouse ASAH2 (NP_061300.1) precursor was expressed with a N-terminal His tag.
Source HEK293
Predicted N Terminal His
AA Sequence Thr 34-Thr 756
Molecular Weight The secreted recombinant mouse ASAH2 consists of 739 a.a. and has a calculated molecular mass of 82 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 105-115 kDa protein in SDS-PAGE under reducing conditions.
Purity >97% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid.The specific activity is > 3,000 pmoles/min/ug.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed