Recombinant Mouse Artemin Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0273
Recombinant Mouse Artemin Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0273
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | Fc |
| Host Species | Mouse |
| Accession | NP_033841.1 |
| Synonym | neublastin |
| Background | Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of- ARTN- action is via binding to a non-signaling co-receptor. The major function of- ARTN- is to drive the molecule to induce migration and axonal projection from sympathetic neurons. It also promotes the survival, proliferation and neurite outgrowth of sympathetic neurons in vitro.- ARTN- triggers oncogenicity and metastasis by the activation of the AKT signaling pathway. Recent studies have reported that the expression of- ARTN- in hepatocellular carcinoma is associated with increased tumor size, quick relapse and shorter survival. Furthermore,- ARTN- promotes drug resistance such as antiestrogens, doxorubicin, fulvestrant, paclitaxel, tamoxifen and trastuzumab. Moreover,- ARTN- also stimulates the radio-therapeutic resistance. Hypoxia has been reported to regulate the cancer stem cell (CSC) population yet the underlying mechanism is poorly characterized. Artemin (ARTN) is a member of the glial cell derived neurotrophic factor family of ligands, is a hypoxia-responsive factor and is essential for hypoxia-induced CSC expansion in hepatocellular carcinoma (HCC). Clinically, elevated expression of ARTN in HCC was associated with larger tumor size, faster relapse and shorter survival. In vitro, HCC cells with forced expression of ARTN exhibited reduced apoptosis, increased proliferation, epithelial-mesenchymal transition (EMT) and enhanced motility. Additionally, ARTN dramatically increased xenograft tumor size and metastasis in vivo. Moreover, ARTN also enhanced tumorsphere formation and the tumor initiating capacity of HCC cells, consequent to expansion of the CD133+ CSC population. ARTN transcription was directly activated by hypoxia-induced factor-1alpha (HIF-1alpha) and hypoxia induced ARTN promoted EMT and increased the CSC population via AKT signaling. |
| Description | A DNA sequence encoding the mouse Artn (NP_033841.1) (Ala112-Gly224) was expressed with the Fc region of human IgG1 at the N-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Glu |
| AA Sequence | Ala112-Gly224 |
| Molecular Weight | The recombinant mouse Artn consists 373 a.a. and predicts a molecular mass of 40.6 kDa. |
| Purity | >90% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
