Recombinant Mouse ARSA Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0265
Recombinant Mouse ARSA Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0265
Catalog No.: BLPSN-0265
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | NP_033843.2 |
| Synonym | As-2, AS-A, As2, ASA, AW212749, TISP73 |
| Background | Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer. |
| Description | A DNA sequence encoding the extracellular domain (Met 1-Ser 506) of mouse ARSA (NP_033843.2) precursor was expressed with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Ser 18 |
| AA Sequence | Met 1-Ser 506 |
| Molecular Weight | The secreted recombinant mouse ARSA consists of 500 a.a. and has a calculated molecular mass of 53.5 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60 kDa protein in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to cleave p-Nitrocatechol Sulfate (PNCS). The specific activity is >100 pmoles/min/ug. |
| Formulation | Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
