Recombinant Mouse Aminoacylase 1 / ACY1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0176
Recombinant Mouse Aminoacylase 1 / ACY1 Protein (His Tag)

Recombinant Mouse Aminoacylase 1 / ACY1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0176
Catalog No.: BLPSN-0176

Product Overview

Tag His
Host Species Mouse
Accession Q99JW2
Synonym 1110014J22Rik, Acy-1
Background Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
Description A DNA sequence encoding the mouse ACY1 (Q99JW2) (Met 1-Ser 408) was expressed with a C-terminal His tag.
Source HEK293
Predicted N Terminal Gln 18
AA Sequence Met 1-Ser 408
Molecular Weight The recombinant mouse ACY1 consists of 402 a.a. and has a calculated molecular mass of 45 kDa as estimated by SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to cleave N-acetyl-L-Methione (Ac-Met). The specific activity is >4,000 pmoles/min/ug.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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