Recombinant Mouse alanyl-tRNA synthetase / CMT2N Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0126
Recombinant Mouse alanyl-tRNA synthetase / CMT2N Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0126
Catalog No.: BLPSN-0126
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | Q8BGQ7 |
| Synonym | AI316495, C76919, sti |
| Background | Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. |
| Description | A DNA sequence encoding the mouse AARS (Q8BGQ7) (Met 1-Asn 968) was fused with a His tag at the C-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met 1 |
| AA Sequence | Met 1-Asn 968 |
| Molecular Weight | The secreted recombinant mouse AARS consists of 978 a.a. and has a calculated molecular mass of 108.3 kDa. The recombinant protein migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >88% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
