Recombinant Mouse 14-3-3 epsilon Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0006
Recombinant Mouse 14-3-3 epsilon Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0006
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | GST |
| Host Species | Mouse |
| Accession | P62259 |
| Synonym | AU019196 |
| Background | YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 1% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner. |
| Description | A DNA sequence encoding the mouse YWHAE (P62259) (Met 1-Gln 255) was fused with the GST tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Gln 255 |
| Molecular Weight | The recombinant mouse YWHAE/GST chimera consists of 487 a.a. and has a predicted molecular mass of 56.1 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 50mM Tris, 2mM GSH, 1mM DTT, pH 8.2. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
