Name: Recombinant Human VLDL Receptor Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4828
Availability: InStock

Recombinant Human VLDL Receptor Protein (His Tag)

Catalog No.: BLPSN-4828

Product Overview

Tag	His
Host Species	Human
Accession	NP_003374.3
Synonym	CAMRQ1, CARMQ1, CHRMQ1, VLDLRCH
Background	The very low density lipoprotein receptor, known as VLDLR, is a single-pass type 1 integral membrance protein and a member of the LDL receptor family. This receptor family includes LDL receptor, LRP, megalin, VLDLR and ApoER2, and is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR contains 3 EGF-like domains, 8 LDL-receptor class A domains, as well as 6 LDL-receptor class B repeats, and is abundant in heart, skeletal muscle, also ovary and kidney, but not in liver. VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR mediates the phosphorylation of mDab1 (mammalian disabled protein) via binding to Reelin, and induces the modulation of Tau phosphorylation. This pathway regulates the migration of neurons along the radial glial fiber network during brain development. Defects of VLDLR may be the cause of VLDLR-associated cerebellar hypoplasia (VLDLRCH), a syndrome characterized by moderate-to-profound mental retardation, delayed ambulation, and predominantly truncal ataxia.
Description	A DNA sequence encoding the human VLDLR isoform alpha (NP_003374.3) extracellular domain (Met 1-Ser 797) was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Gly 28
AA Sequence	Met 1-Ser 797
Molecular Weight	The recombinant human VLDLR comprises 781 a.a. with a predicted molecular mass of 86 kDa. It migrates with the apparent molecular weight of 150 & 180 kDa due to different glycosylation in SDS-PAGE under reducing conditions.
Purity	>80% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1.Measured by its binding ability in a functional ELISA.2.Immobilized human VLDLR-His at 10ug/mL (100uL/well) can bind biotinylated human LRPAP1-His, the EC50 of biotinylated human LRPAP1-His is 0.05-0.2 ug/mL.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.