Recombinant Human VLDL Receptor Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4828
Recombinant Human VLDL Receptor Protein (His Tag)

Recombinant Human VLDL Receptor Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4828
Catalog No.: BLPSN-4828

Product Overview

Tag His
Host Species Human
Accession NP_003374.3
Synonym CAMRQ1, CARMQ1, CHRMQ1, VLDLRCH
Background The very low density lipoprotein receptor, known as VLDLR, is a single-pass type 1 integral membrance protein and a member of the LDL receptor family. This receptor family includes LDL receptor, LRP, megalin, VLDLR and ApoER2, and is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR contains 3 EGF-like domains, 8 LDL-receptor class A domains, as well as 6 LDL-receptor class B repeats, and is abundant in heart, skeletal muscle, also ovary and kidney, but not in liver. VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR mediates the phosphorylation of mDab1 (mammalian disabled protein) via binding to Reelin, and induces the modulation of Tau phosphorylation. This pathway regulates the migration of neurons along the radial glial fiber network during brain development. Defects of VLDLR may be the cause of VLDLR-associated cerebellar hypoplasia (VLDLRCH), a syndrome characterized by moderate-to-profound mental retardation, delayed ambulation, and predominantly truncal ataxia.
Description A DNA sequence encoding the human VLDLR isoform alpha (NP_003374.3) extracellular domain (Met 1-Ser 797) was fused with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Gly 28
AA Sequence Met 1-Ser 797
Molecular Weight The recombinant human VLDLR comprises 781 a.a. with a predicted molecular mass of 86 kDa. It migrates with the apparent molecular weight of 150 & 180 kDa due to different glycosylation in SDS-PAGE under reducing conditions.
Purity >80% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1.Measured by its binding ability in a functional ELISA.2.Immobilized human VLDLR-His at 10ug/mL (100uL/well) can bind biotinylated human LRPAP1-His, the EC50 of biotinylated human LRPAP1-His is 0.05-0.2 ug/mL.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed