Recombinant Human Vinculin Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4813
Recombinant Human Vinculin Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4813
Catalog No.: BLPSN-4813
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P18206 |
| Synonym | CMD1W, CMH15, HEL114, MV, MVCL |
| Background | Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin. |
| Description | A DNA sequence encoding the human VCL (P18206-2) (Met 1-Gln 1066) was expressed, fused with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Met 1 |
| AA Sequence | Met 1-Gln 1066 |
| Molecular Weight | The recombinant human VCL consists of 1077 a.a. and has a calculated molecular mass of 118 kDa. The apparent molecular mass of rhVCL is approximately 115 kDa in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
