Name: Recombinant Human Vimentin Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4812
Availability: InStock

Recombinant Human Vimentin Protein (His Tag)

Catalog No.: BLPSN-4812

Product Overview

Tag	His
Host Species	Human
Accession	P08670
Synonym	CTRCT30, HEL113
Background	Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central alpha-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.
Description	A DNA sequence encoding the human VIM (Met 1-Glu466) (P08670) was expressed, with a C-terminal His tag.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Met
AA Sequence	Met 1-Glu466
Molecular Weight	The secreted recombinant human VIM consists of 476 a.a. and predicts a molecular mass of 55 KDa. The apparent molecular mass of the protein is approximately 56 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 40% acetonitrile, 0.1% TFA.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.