Recombinant Human VCAM1 Protein
Beta LifeScience
SKU/CAT #: BL-4906PS
Recombinant Human VCAM1 Protein
Beta LifeScience
SKU/CAT #: BL-4906PS
Catalog No.: BL-4906PS
Product Overview
| Tag | His |
| Host Species | Human |
| Synonym | Vascular Cell Adhesion Molecule 1, CD106 Antigen,INCAM-100 , Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, L1CAM. |
| Background | VCAM1 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 contains 6 or 7 immunoglobulin domains, and is expressed on both large and small vessels only after the endothelial cells are stimulated by cytokines. The protein has a number of functions including the regulation of leukocyte migration, leukocyte endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases (artherosclerosis and rheumatoid arthritis). |
| Description | VCAM1 expressed in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 685a.a. (25-698 a.a.) andhaving a molecular weight of 75.6kDa (Molecular size on SDS-PAGE will appear atapproximately 70-100kDa). VCAM1 is expressed with a 11a.a. His tag atC-Terminus and purified by unique purification methods. |
| Source | Sf9 |
| AA Sequence | ADPEFFKIET TPESRYLAQI GDSVSLTCSTTGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDPEIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDIGKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWSKKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGDSVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVELYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMTFIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGFPAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFPSESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLRSLTLDVQGRE NNKDYFSPEH HHHHH. |
| Purity | Greaterthan 90.0% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | VCAM1 protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
