Recombinant Human VCAM1 Protein

Beta LifeScience SKU/CAT #: BL-4906PS

Recombinant Human VCAM1 Protein

Beta LifeScience SKU/CAT #: BL-4906PS
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag His
Host Species Human
Synonym Vascular Cell Adhesion Molecule 1, CD106 Antigen,INCAM-100 , Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, L1CAM.
Background VCAM1 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 contains 6 or 7 immunoglobulin domains, and is expressed on both large and small vessels only after the endothelial cells are stimulated by cytokines. The protein has a number of functions including the regulation of leukocyte migration, leukocyte endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases (artherosclerosis and rheumatoid arthritis).
Description VCAM1 expressed in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 685a.a. (25-698 a.a.) andhaving a molecular weight of 75.6kDa (Molecular size on SDS-PAGE will appear atapproximately 70-100kDa). VCAM1 is expressed with a 11a.a. His tag atC-Terminus and purified by unique purification methods.
Source Sf9
AA Sequence ADPEFFKIET TPESRYLAQI GDSVSLTCSTTGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDPEIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDIGKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWSKKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGDSVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVELYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMTFIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGFPAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFPSESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLRSLTLDVQGRE NNKDYFSPEH HHHHH.
Purity Greaterthan 90.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation VCAM1 protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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