Recombinant Human USP7 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4754
Recombinant Human USP7 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4754
Catalog No.: BLPSN-4754
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | NP_003461.2 |
| Synonym | HAUSP, TEF1 |
| Background | Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein which belongs to thepeptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP stimulates lytic infection and the reactivation of quiescent viral genomes. ICP interacts very strongly with USP7. USP7-mediated stabilization of ICP is dominant over ICP-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP are expressed. |
| Description | A DNA sequence encoding full length of human USP7 (NP_003461.2) (Met 1-Asn 1102) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Asn 1102 |
| Molecular Weight | The recombinant human USP7/GST chimera consists of 1339 a.a. and has a calculated molecular mass of 156 kDa. It migrates as an approximately 140 band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 50mM Tris 100mM NaCl, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
