Recombinant Human uPAR Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4742
Recombinant Human uPAR Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4742
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | Q03405 |
| Synonym | CD87, U-PAR, UPAR, URKR |
| Background | Urokinase plasminogen activator (uPA) and/or its receptor (uPAR) are essential for metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumours. uPAR and uPA levels in both resected tumor tissue and plasma are of independent prognostic significance for patient survival in several types of human cancer. This system has classically been thought to drive tumor progression by mediating directed extracellular proteolysis on the surface of migrating or invading cells, and intervening with this proteolysis by targeting uPAR has been proposed to represent a novel approach for inhibiting tumor progression. uPAR, also known as PLAUR or CD87, has been implicated in the growth, metastasis, and angiogenesis of several solid and hemotologic malignancies. uPAR is a highly glycosylated, 55-6kDa integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It is part of a cell surface system that also consists of the serine protease uPA and several specific inhibitors (plasminogen activator inhibitors 1 and 2). Additionally, the analysis of CD87 (urokinase-type plasminogen activator receptor - uPAR) expression has a potential role in the diagnostic or prognostic work-up of several hematological malignancies, particularly acute leukemia and multiple myeloma. |
| Description | A DNA sequence encoding the human UPAR isoform 1 (Q03405-1) (Met 1-Arg 303) without the pro peptide was expressed, with a carboxy-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Leu 23 |
| AA Sequence | Met 1-Arg 303 |
| Molecular Weight | The secreted recombinant human UPAR consists of 292 a.a. with the predicted molecular mass of 32.8 kDa. As a result of glycosylation, rhUPAR migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >98% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human uPAR at 5 ug/ml (100 ul/well) can bind biotinylated human UPA with a linear ranger of 40-1000 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
