Recombinant Human UCHL3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4724
Recombinant Human UCHL3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4724
Catalog No.: BLPSN-4724
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_005993.1 |
| Synonym | UCH-L3 |
| Background | Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to thepeptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer. |
| Description | A DNA sequence encoding the human UCHL3 (NP_005993.1) (Glu 2-Ala 230) was expressed, with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Glu 2-Ala 230 |
| Molecular Weight | The recombinant human UCHL3 consisting of 236 a.a. and has a calculated molecular mass of 27 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14,000 pmoles/min/ug. |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
