Recombinant Human Ube2H Protein
Beta LifeScience
SKU/CAT #: BLPSN-4716

Recombinant Human Ube2H Protein
Beta LifeScience
SKU/CAT #: BLPSN-4716
Catalog No.: BLPSN-4716
Product Overview
Tag | N/A |
Host Species | Human |
Accession | P62256 |
Synonym | E2-20K, GID3, UBC8, UBCH, UBCH2 |
Background | UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. |
Description | A DNA sequence encoding the human UBE2H (P62256) (Met 1-Leu 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus. |
Source | E.coli |
Predicted N Terminal | Gly |
AA Sequence | Met 1-Leu 183 |
Molecular Weight | The recombinant human UBE2H consists of 185 a.a. and migrates as an approximately 21 kDa band in SDS-PAGE under reducing conditions as predicted. |
Purity | >93% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, 10% glycerol, 2mM DTT, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |