Recombinant Human Ube2H Protein

Beta LifeScience SKU/CAT #: BLPSN-4716
Recombinant Human Ube2H Protein

Recombinant Human Ube2H Protein

Beta LifeScience SKU/CAT #: BLPSN-4716
Catalog No.: BLPSN-4716

Product Overview

Tag N/A
Host Species Human
Accession P62256
Synonym E2-20K, GID3, UBC8, UBCH, UBCH2
Background UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
Description A DNA sequence encoding the human UBE2H (P62256) (Met 1-Leu 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
Source E.coli
Predicted N Terminal Gly
AA Sequence Met 1-Leu 183
Molecular Weight The recombinant human UBE2H consists of 185 a.a. and migrates as an approximately 21 kDa band in SDS-PAGE under reducing conditions as predicted.
Purity >93% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, 10% glycerol, 2mM DTT, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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