Recombinant Human TRIB2 Protein (His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-4648
Recombinant Human TRIB2 Protein (His & GST Tag)

Recombinant Human TRIB2 Protein (His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-4648
Catalog No.: BLPSN-4648

Product Overview

Tag His&GST
Host Species Human
Accession NP_006204.1
Synonym C5FW, FLJ57420, GS3955, TRB2, TRIB2
Background Tribbles homolog 2, also known as TRB-2, and Trib2, is a member of the protein kinase superfamily and Tribbles subfamily (Trib1, Trib2, Trib3). The identification of tribbles as regulators of signal processing systems and physiological processes, including development, together with their potential involvement in diabetes and cancer, has generated considerable interest in these proteins. Tribbles have been reported to regulate activation of a number of intracellular signalling pathways with roles extending from mitosis and cell activation to apoptosis and modulation of gene expression. Tribbles controls the timing of mitosis in the prospective mesoderm, allowing cell-shape changes to be completed. This mechanism for coordinating cell division and cell-shape changes may have helped Drosophila to evolve its mode of rapid early development. Trib2 was identified as a downregulated transcript in leukemic cells undergoing growth arrest. Trib2-transduced bone marrow cells exhibited a growth advantage and readily established factor-dependent cell lines. Trib2-reconstituted mice uniformly developed fatal transplantable acute myelogenous leukemia (AML).
Description A DNA sequence encoding the human TRIB2 (NP_006204.1) (Met 1-Asn 343) was fused with the N-terminal His-tagged GST tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Met
AA Sequence Met 1-Asn 343
Molecular Weight The recombinant human TRIB2/GST chimera consists of 580 a.a. and predicts a molecular mass of 66.6 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Purity >82% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM GSH, pH 8.0.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed