Recombinant Human Transferrin Receptor Protein (Fc Tag)

Name: Recombinant Human Transferrin Receptor Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-4631
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Recombinant proteins

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Product Overview

Tag	Fc
Host Species	Human
Accession	CAA25527.1
Synonym	CD71, p90, T9, TFR, TFR1, TR, TRFR
Background	Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein which belongs to thepeptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.
Description	A DNA sequence encoding the human TFRC (CAA25527.1) (Cys89-Phe760) was expressed with the Fc region of human IgG1 at the N-terminus.
Source	HEK293
Predicted N Terminal	Glu
AA Sequence	Cys89-Phe760
Molecular Weight	The recombinant human TFRC consists of 932 a.a. and predicts a molecular mass of 103.6 kDa.
Purity	>87% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2.; (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Subcellular Location	Cell membrane; Single-pass type II membrane protein. Melanosome.; [Transferrin receptor protein 1, serum form]: Secreted.
Protein Families	Peptidase M28 family, M28B subfamily
Database References	HGNC: 11763 OMIM: 190010 KEGG: hsa:7037 STRING: 9606.ENSP00000353224 UniGene: Hs.529618
Associated Diseases	Immunodeficiency 46 (IMD46)

Gene Functions References

TFR1 was increased in myelodysplastic syndrome patients and expression was upregulated in CD34+ cells from patients with refractory anaemia with ringed sideroblasts (RARS). PMID: 28714470
palmitoylation is reported as a hitherto unreported level of post-translational TfR1 regulation. PMID: 29395073
Our data provide evidence that blocking TfR could significantly inhibit lung adenocarcinoma (LAC) proliferation by targeting the oncogene KRAS; therefore, TfR may be a therapeutic target for LAC. In addition, our results suggest a new method for blocking the signal from the oncogene KRAS by targeting TfR in LAC. PMID: 29286585
TfR could be used as a marker of erythropoiesis in high-flux hemodialysis patients PMID: 28446125
The results suggest a role of microRNAs in the TfR1 regulation in the iron-regulatory protein-iron-responsive element system. PMID: 29295890
Cell viability and surface expression of transferrin receptor (CD71) and glycophorin A (GPA) were analyzed before and after re-culture by flow cytometry. These studies show differential sensitivities of these surface proteins on K562 cells to proteases, and suggest molecular mechanisms of transmembrane protein transport and cycling. PMID: 28980921
These data show that TfR1-PvRBP2b invasion pathway is critical for the recognition of reticulocytes during P. vivax invasion. PMID: 29302006
combination of desferal with oxaliplatin can overcome cervical cancer oxaliplatin resistance through the regulation of hCtr1 and TfR1 PMID: 27384479
Data suggest that hematologic parameters in children consuming lacto-ovo vegetarian diets are comparable with those of control children, but ferritin levels are lower; inclusion of novel serum biomarkers, soluble transferrin receptor and hepcidin, in nutritional assessment can better detect subclinical iron deficiency in children following vegetarian diet. This study was conducted in Poland with children ages 4.5-9 years. PMID: 28342014
results suggest that lower Hepcidin-25, as well as higher sTfR and sTfR/Hepcidin-25 ratio were significant predictors of favorable hemoglobin response within a month after IV administration of ferric carboxymaltose in patients with CKD PMID: 27282576
TfR1 was highly expressed in glioblastomas and associated with shorter survival in the whole cohort, but not in the individual malignancy grades PMID: 28837569
Expression of Hepcidin and Ferroportin in the Placenta, and Ferritin and Transferrin Receptor 1 Levels in Maternal and Umbilical Cord Blood in Pregnant Women with and without Gestational Diabetes PMID: 27483296
Human melanoma cells are able to up-regulate TFRC expression using hyaluronan/CD44 signaling. PMID: 28551638
the group with homozygous or compound heterozygous state for beta-thalassemia had the highest sTfR1 levels and that the presence of increased sTfR1 levels (>5 times normal) was associated with a complex and severe history of disease requiring splenectomy, occasional red blood cells transfusions, and early start and continuous iron chelation therapy PMID: 28707012
high expression of transferrin receptor-1 resulting in iron uptake contributes to increase in the labile iron pool which plays roles in cholangiocarcinoma progression with aggressive clinical outcomes. PMID: 28671021
EGFR regulates iron homeostasis to promote non-small cell lung cancer growth through redistribution of transferrin receptor 1. PMID: 27523281
TFR1 is overexpressed in adult T-cell leukemia/lymphoma caells . PMID: 28189691
NSF deficiency in HeLa cells barely affected cell viability, anterograde trafficking of vesicular stomatitis virus glycoprotein G and transferrin endocytosis. PMID: 27995606
inhibition of c-Abl minimizes receptor recycling pathways and results in chaperone-dependent trafficking of the TfR1 to the lysosome for degradation. PMID: 27226592
alpha6(high)/CD71(low) cells give rise to a thicker pluristratified epithelium with lower seeding density and display a low Ki67 positive cells number, showing that they have reached the balance between proliferation and differentiation. PMID: 28134816
Data show that the concentration of soluble transferrin receptor with high diagnostic value in the detection of iron deficiency-based anemia in patients undergoing dialysis for chronic renal failure at the end-stage. PMID: 27629729
inverse correlation between the total percentage of CD71-positive NRBCs and gestational age suggests that this analysis may help in pregnancy dating PMID: 25906120
Data indicate an over-expression of transferrin receptor 1 (TFRC) in hepatocellular carcinoma (HCC) and a markedly decreased level of microRNA-152 (miR-152) when compared to non-tumor liver tissue. PMID: 26657500
Together, our findings reveal that TfR1 can contribute to the mitochondrial respiration and ROS production, which have essential roles in growth and survival of pancreatic cancer. PMID: 26869514
siRNA targeted delivery using transferrin-coupled lipoplexes specifically sensitizes CD71 high expressing malignant cells to antibody-mediated complement attack PMID: 25395366
Case Report: cellular iron deficit due to the low level of transferrin receptor, particularly in erythroid tissue. PMID: 26339443
The requirement for a threshold level of TFR1 expression can explain why among primary human hematopoietic cells, only erythroblasts efficiently take up H-ferritin. PMID: 26441243
Data indicate only the glycoproteins CD34+/CD71low population showed statistically significant differences between Diamond-Blackfan Anaemia (DBA) patients and controls. PMID: 26394034
These results demonstrate that in cells expressing the hTfR, Tacaribe virus internalization depends on the presence of cholesterol, dynamin and acidic intracellular vesicles. PMID: 26559962
The importance of TfR1 in adaptive immunity.A missense mutation in TFRC, encoding transferrin receptor 1, causes combined immunodeficiency. PMID: 26642240
The blood levels of TfRC was higher in age-related macular degeneration patients than controls.No association was found between age-related macular degeneration occurrence and the p.Gly142Ser polymorphism of the TRFC gene. PMID: 25915522
Data show that interleukin 6 (IL-6) secretion and the percentage of CD71 antigen-positive T lymphocytes both uniquely predicted survival independent of papillomavirus infection status. PMID: 26079381
This study provides insights into the effector functions of human IgG3 in the context of an antibody targeting TfR1. PMID: 26232328
this report provided a convenient protocol that could be fulfilled in order to prepare hTfR1 inclusion body, which failed to be purified by an Ni(2+) affinity column. PMID: 25207990
elucidation of a signalling pathway whereby C18:0 stearoylates TFR1, thereby inhibiting its activation of JNK signaling; this leads to reduced ubiquitination of mitofusin via HUWE1, thereby promoting mitochondrial fusion and function PMID: 26214738
The surface levels of three receptors [TfR, epidermal growth factor receptor (EGFR) and beta1 integrin] were tested and found to be reduced dependent on Escherichia coli EspG translocation. PMID: 24898821
High TFRC expression is associated with response to low intravenous immunoglobulin therapy in patients with Kawasaki disease. PMID: 25449331
The rate of TFRC expression in oral sqamous cell neoplasms was significantly higher than that in dysplasia, suggesting that it may be involved in disease progression. PMID: 24890018
CD71 is useful for the evaluation of differentiation stage for AML cells. PMID: 23962073
these findings suggest that TFR1 has an important role in sphingosine kinase 1-mediated oncogenesis. PMID: 24276247
a critical review the available literature to assess the diagnostic efficacy of sTfR in complicated anemia--{REVIEW} PMID: 24525213
Expression of the transferrin receptor CD71 is associated with clinical features of esophageal squamous cell carcinoma. PMID: 24435655
results suggest that Transferrin or a TF-TFR2 complex may have a role in the etiology of PD, possibly through iron misregulation or mitochondrial dysfunction within dopaminergic neurons. PMID: 24121126
high levels of TfRs such as those found on activated lymphocytes were found to be associated with decreased KLRG1 inhibitory function, indicating that TfRs may sequester KLRG1 from interacting with cadherins. PMID: 24752778
MARCH 8 mediates the ubiquitination and lysosomal degradation of the transferrin receptor. PMID: 23606747
A statistically significant difference in urinary sTfR concentration was observed between patients with active IgA nephropathy or Henoch-Schonlein purpura nephritia and patients who had achieved partial or complete remission PMID: 23384534
The kinesin KIF16B mediates apical transcytosis of transferrin receptor in AP-1B-deficient epithelia. PMID: 23749212
Knockdown of Rab12 increased transferrin receptor level and reduced M98K-induced cell death. PMID: 23357852
TfR1 plays a role in hepatitis C virus (HCV) infection at the level of glycoprotein-mediated entry, acts after CD81, and possibly is involved in HCV particle internalization. PMID: 23754414
Among the PC family members, only furin activates hepcidin in hepatocytes, and uniquely the full-length membrane-bound PC7 can directly shed hTfR1 by cleavage at Arg100 PMID: 23390091

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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