Recombinant Human TPK1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4613
Recombinant Human TPK1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4613
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | P02775 |
Synonym | HTPK1, PP20, THMD5 |
Background | Thiamine pyrophosphokinase (TPK) produces thiamine pyrophosphate, a cofactor for a number of enzymes, including pyruvate dehydrogenase and 2-ketoglutarate dehydrogenase. Episodic encephalopathy type thiamine metabolism dysfunction (OMIM 614458) due to TPK1 mutations is a recently described rare disorder. The genomic variations in the fetal and maternal TPK1 gene could contribute to the variability of birth weight in normal humans. |
Description | A DNA sequence encoding the human TPK1 (AAH68460.1) (Met1-Ser243) was expressed with a His tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | His |
AA Sequence | Met1-Ser243 |
Molecular Weight | The recombinant human TPK1 consists of 261 a.a. and predicts a molecular mass of 29.5 KDa. It migrates as an approximately 27-32 KDa band in SDS-PAGE under reducing conditions. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. |
Subcellular Location | Secreted. |
Protein Families | Intercrine alpha (chemokine CxC) family |
Database References |
Gene Functions References
- Low CXCL7 expression is associated with posterior longitudinal ligament ossification. PMID: 29782494
- In the prospective population-based Atherosclerosis Risk in Communities cohort, beta-thromboglobulin was not associated with cardiovascular disease risk. PMID: 28531882
- Cleavage of a factor Xa-specific chromogenic substrate was observed when Beta-TG was incubated with factor X, suggesting a direct interaction between Beta-TG and factor X. Beta-TG modulate coagulation in vitro via an interaction with factor X. PMID: 28384443
- This study demonstrated that platelet basic protein was detected in the serums of Alzheimer disease patients PMID: 27911324
- DEFA1, DEFA3, and PPBP expression was significantly increased in hyperlipidemia and coronary heart disease patients compared with controls. PMID: 28420383
- These results suggest that aberrant CXCL7 expression in peripheral blood cells is induced by renal cell carcinoma cells and may serve as a novel biomarker in the diagnosis of renal cell carcinoma . PMID: 28985012
- Two haplotype blocks, one upstream to the coding region of UGT2A1 (rs146712414, P = 9.1 x 10(-5); odds ratio [OR], 1.34; 95% confidence interval [CI], 1.16-1.56) and one downstream of the genes PF4/PPBP/CXCL5 (rs1595009, P = 1.3 x 10(-4); OR, 1.32; 95% CI, 1.15-1.52), were associated with AgP. PMID: 28467728
- NMR and modeling studies indicated that glycosaminoglycans (GAG) heparin binding to the heterodimer is distinctly different from the CXC chemokine ligand 7 (CXCL7) monomer. PMID: 28368308
- Cby's C-terminal domain alone binds to TC-1 with significantly greater affinity compared to full-length Cby, implying that target binding of the coiled-coil domain is affected by the flanking disordered regions. PMID: 27082063
- CXCL7 plays a promoting role in regulating the growth and metastasis of cholangiocarcinoma. PMID: 27959418
- Data suggest that neutrophil-activating peptide 2 (NAP-2) secreted by NK cells can bind to CXC Chemokine Receptor 2 (CXCR2) on mesenchymal stem cells (MSCs) leading to stimulation of its recruitment. PMID: 27052313
- Utilizing mass spectrometry-based proteomics, platelet basic protein has been identified as a candidate serum biomarker for transient ischemic attack PMID: 26307429
- Synovial Cxcl7 mRNA and protein were increased in early rheumatoid arthritis compared to uninflamed controls and resolving arthritis. PMID: 25858640
- Studied and characterized the dynamics and thermodynamic stability of monomer and homodimer structures of CXCL7. PMID: 26297927
- Leptin and neutrophil-activating peptide act synergistically to promote mesenchymal stem cell senescence through enhancement of the PI3K/Akt signaling pathway in systemic lupus erythematosus patients. PMID: 25989537
- CXCL7/CXCR2 signalling pathways are a predictive factor of poor outcome in metastatic colon cancer. PMID: 25580640
- CXCR4-CXCL12-CXCR7 regulates mTOR signaling in renal cancer cells. PMID: 24991762
- The antimicrobial thrombocidin peptides are found in human blood platelets, are deletion products of CXC chemokines and have antibacterial and antifungal activity. PMID: 10877842
- The level of beta-thromboglobulin was found significantly higher in female patients with rheumatoid arthritis. PMID: 23052484
- Testing of bacterial resistance to platelet antimicrobial lmw proteins (PMPs) may be advisable as a predictive indicator of susceptibility to treatment of infections such as infective endocarditis and other local infections of biofilm nature. PMID: 23619231
- beta-TG was evaluated for familial mediterranean fever cases and healthy controls, beta-TG levels were found significantly lower among patients; it is hypothesized that this difference may have resulted from the effect of colchicine use on platelet functions. PMID: 22939812
- Examined the prognostic values of SARS-associated proteome, and deciphered the identities of those with prognostic values.The associations of decreased serum PF4 and increased serum beta-TG levels with poor prognosis were confirmed by Western blot. PMID: 22740477
- modulates antimicrobial activity of phagocytes against L. pneumophila PMID: 22101183
- positive ratios of CXCR7, CXCL12 and CXCL11 in oral leukoplakia and oral squamous cell carcinoma tissues respectively, were significantly higher than that in normal epithelia PMID: 21442287
- Using microdialysis techniques we detected beta-TG release during an inflammatory response. We conclude that local platelet activation is induced by the heating stimulus. PMID: 20510637
- beta-TG is cleaved after being released from activated platelets, thereby becoming less mitogenic for fibroblasts PMID: 20224257
- CXCL7 promotes neutrophil adhesion to vascular endothelium and induces transendothelial migration. PMID: 12193731
- one or more high molecular weight protein is released from alpha-granules and is broken down into smaller fragments after release to form beta-thromboglobulin (beta-TG)-like proteins by the action of metal dependent proteases. PMID: 12297130
- Gene profiling iodentified PPBP from peripheral blood cells in coronary artery disease. PMID: 12878486
- Platelet basic protein is downregulated by glucocorticoids, and is defined as an immunosuppressive target of glucocorticoids. PMID: 14673015
- Our observations indicate that PBP and its derivates are constituents of the antimicrobial arsenal of human monocytes. Their increased expression after exposure to microorganisms allows a rapid host response to pathogens. PMID: 15316029
- activated human skin mast cells (MCs) convert CTAP-III into biologically active NAP-2 through proteolytic cleavage by released chymase. PMID: 16317101
- stromal-stimulated monocytes can serve as an additional source for CXCL7 peptides in the microenvironment and may contribute to the local regulation of megakaryocytopoiesis PMID: 16391012
- levels of BDNF and beta-TG in blood of Alzheimers patients is decreased; BDNF and beta-TG are associated with degree of platelet activation PMID: 16807663
- NAP-2 has the potential to induce inflammatory responses within the atherosclerotic plaque. By its ability to promote leukocyte and endothelial cell activation, such a NAP-2-driven inflammation could promote plaque rupture and acute coronary syndromes. PMID: 17045893
- CXCL7 decreased serum levels in advanced MDS, suggesting the possibility of a concerted disturbance of transcription or translation of these chemokines in advanced MDS PMID: 17220270
- delayed pressure urticaria may be associated with increased secretion of platelet chemokines PF4 and beta-TG, similar to that observed in cold urticaria PMID: 18632422
- stimulates the migration of mesenchymal stem cells in vitro PMID: 18707017