Recombinant Human TMED1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4559
Recombinant Human TMED1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4559
Catalog No.: BLPSN-4559
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | Q13445 |
| Synonym | Il1rl1l, IL1RL1LG, Tp24 |
| Background | TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side. |
| Description | A DNA sequence encoding the human TMED1 (Q13445) (Met1-Asn194) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Ala 24 |
| AA Sequence | Met1-Asn194 |
| Molecular Weight | The recombinant human TMED1 consists of 182 a.a. and predicts a molecular mass of 20.6 KDa. It migrates as an approximately 28 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | 1.Measured by its binding ability in a functional ELISA.2.Immobilized human TMED1-His at 10ug/mL (100uL/well) can bind human IL1R4-Fc, the EC50 of human IL1R4-Fc is 8-50ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
