Recombinant Human TMED1 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-4558
Recombinant Human TMED1 Protein (Fc Tag)

Recombinant Human TMED1 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-4558
Catalog No.: BLPSN-4558

Product Overview

Tag Fc
Host Species Human
Accession Q13445
Synonym Il1rl1l, IL1RL1LG, Tp24
Background TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.
Description A DNA sequence encoding the human TMED1 (Q13445) (Met1-Asn194) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Ala 24
AA Sequence Met1-Asn194
Molecular Weight The recombinant human TMED1 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 412 a.a. and has a predicted molecular mass of 46.2 kDa. The apparent molecular mass of the protein is approximately 57 kDa in SDS-PAGE under reducing conditions.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1.Measured by its binding ability in a functional ELISA.2.Immobilized mouse ST2-His at 10ug/mL (100uL/well) can bind human TMED1-Fc, the EC50 of human TMED1-Fc is 0.05-0.2ug/mL.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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