Name: Recombinant Human TLR4 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4550
Availability: InStock

Recombinant Human TLR4 Protein (His Tag)

Catalog No.: BLPSN-4550

Product Overview

Tag	His
Host Species	Human
Accession	O00206
Synonym	ARMD10, CD284, TLR-4, TLR4, TOLL
Background	TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).
Description	A DNA sequence encoding the human TLR4 (Met 1-Lys631) (O00206-1) was expressed, with a C-terminal His tag.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Glu 24
AA Sequence	Met 1-Lys631
Molecular Weight	The secreted recombinant human TLR4 consists of 619 a.a. and predicts a molecular mass of 70.5 KDa. The apparent molecular mass of the protein is approximately 68 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>87% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.