Recombinant Human TBC1D22B Protein

Beta LifeScience SKU/CAT #: BL-4270PS

Recombinant Human TBC1D22B Protein

Beta LifeScience SKU/CAT #: BL-4270PS
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag N/A
Host Species Human
Synonym TBC1 Domain Family, Member 22B, C6orf197, Chromosome 6Open Reading Frame 197, TBC1 Domain Family Member22B, TBC1 domain family member 22B.
Background TBC1 domain family member 22B, also known as TBC1D22B acts as a GTPase-activating protein for Rab family protein. The Tre-2/Bub2/Cdc16 (TBC) domainis a conserved protein motif which consists of roughly 200aa andfunctions as a specific Rab-GAP domain. The TBC domain has more than 40 distinctTBC domain-containing proteins which have been identified in humans.
Description TBC1D22B Human Recombinant expressed in E.Coli is asingle, non-glycosylated polypeptide chain containing 528a.a. (1-505 a.a)and having a molecular weight of 61.5kDa.TBC1D22B is fused to a 23a.a. His-tag atN-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMAAENSK QFWKRSAKLP GSIQPVYGAQ HPPLDPRLTK NFIKERSKVNTVPLKNKKAS SFHEFARNTS DAWDIGDDEE EDFSSPSFQT LNSKVALATA AQVLENHSKL RVKPERSQSTTSDVPANYKV IKSSSDAQLS RNSSDTCLRN PLHKQQSLPL RPIIPLVARI SDQNASGAPP MTVREKTRLEKFRQLLSSQN TDLDELRKCS WPGVPREVRP ITWRLLSGYL PANTERRKLT LQRKREEYFG FIEQYYDSRNEEHHQDTYRQ IHIDIPRTNP LIPLFQQPLV QEIFERILFI WAIRHPASGY VQGINDLVTP FFVVFLSEYVEEDVENFDVT NLSQDMLRSI EADSFWCMSK LLDGIQDNYT FAQPGIQKKV KALEELVSRI DEQVHNHFRRYEVEYLQFAF RWMNNLLMRE LPLRCTIRLW DTYQSEPEGF SHFHLYVCAA FLIKWRKEIL DEEDFQGLLMLLQNLPTIHW GNEEIGLLLA EAYRLKYMFA DAPNHYRR.
Purity >80% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation TBC1D22B protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4) 30% glycerol and 1mM DTT.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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