Recombinant Human SNURF Protein

Beta LifeScience SKU/CAT #: BL-4219PS
Recombinant Human SNURF Protein

Recombinant Human SNURF Protein

Beta LifeScience SKU/CAT #: BL-4219PS
Catalog No.: BL-4219PS

Product Overview

Tag N/A
Host Species Human
Synonym SNRPN Upstream Reading Frame Protein.
Background SNURF is an extremely basic protein restricted to the nucleus. The evolutionarily reserved open reading frame is located on a bicistronic transcript which has a downstream ORF encoding the small nuclear ribonucleoprotein polypeptide N. The first three exons of the transcript are exploited by the upstream coding region which is known as an imprinting center. The full-length nature of these transcripts is yet to be determined but multiple transcription initiation sites have been identified and large scale alternative splicing takes place in the 5' untranslated region. An alternate exon which substitutes for exon 4 and leads to a truncated, monocistronic transcript was identified. Deletion or alternative splicing expressed by a translocation event in the 5' UTR or coding region of this gene results in Prader-Willi syndrome or Angelman syndrome because of parental imprint switch failure.
Description SNURF Human Recombinant expressed in E.coli is a single, non-glycosylated polypeptide chain containing 94a.a. (1-71) and having a molecular weight of 10.8 kDa. SNURF is fused to a 23a.a. His-tag at N-terminus.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMERARDR LHLRRTTEQH VPEVEVQVKR RRTASLSNQE CQLYPRRSQQ QQVPVVDFQA ELRQAFLAET PRGG
Purity >90% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The SNURF solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 40% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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