Recombinant Human SMARCA4 Protein

Beta LifeScience SKU/CAT #: BL-4208PS
Recombinant Human SMARCA4 Protein

Recombinant Human SMARCA4 Protein

Beta LifeScience SKU/CAT #: BL-4208PS
Catalog No.: BL-4208PS

Product Overview

Tag N/A
Host Species Human
Synonym SWI/SNF Related, Matrix Associated, Actin DependentRegulator Of Chromatin, Subfamily A, Member 4, Mitotic Growth And TranscriptionActivator, ATP-Dependent Helicase SMARCA4, BRG1, Global Transcription ActivatorHomologous Sequence Sucrose Nonfermenting-Like 4, BRG1-Associated Factor 190A,Protein Brahma Homolog 1, BRM/SWI2-Related Gene 1, Homeotic Gene Regulator, BrahmaProtein-Like 1 Nuclear Protein GRB1, Protein BRG-1, SNF2-Like 4, SNF2-Beta,BAF190A, SNF2L4, MRD16, RTPS2, SWI/SNF-Related Matrix-AssociatedActin-Dependent Regulator Of Chromatin Subfamily A Member 4, TranscriptionActivator BRG1, EC 3.6.4.-, EC 3.6.1, BAF190, SNF2LB, HSNF2b, SNF2B, SNF2, SWI2,Transcription activator BRG1.
Background SMARCA4 belongs to the SWI/SNF family of proteins and is similar to thebrahma protein of Drosophila.Proteins in this family have helicase and ATPase activities and are consideredto regulate transcription ofcertain genes by altering the chromatin structure around those genes. SMARCA4is part of the large ATP-dependent chromatinremodeling complex SNF/SWI, which is essential for transcriptional activation of genes normally repressed by chromatin. Inaddition, SMARCA4 is able to bind BRCA1, as well as regulating the expressionof thetumorigenic protein CD44. Moreover, mutations in SMARCA4 cause rhabdoid tumorpredisposition syndrome type 2.
Description SMARCA4 Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain containing 211a.a. (362-549a.a.) andhaving a molecular weight of 25kDa.SMARCA4 is fused to a 23a.a. His-tag at N-terminusand purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSDPVEILQ EREYRLQARI AHRIQELENL PGSLAGDLRT KATIELKALRLLNFQRQLRQ EVVVCMRRDT ALETALNAKA YKRSKRQSLR EARITEKLEK QQKIEQERKR RQKHQEYLNSILQHAKDFKE YHRSVTGKIQ KLTKAVATYH ANTEREQKKENERIEKERMR RLMAEDEEGY RKLIDQKKDK R.
Purity >80.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation SMARCA4 protein solution (1mg/ml) containing 20mMTris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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