Recombinant Human SIRT5 Protein

Beta LifeScience SKU/CAT #: BL-4652PS
Recombinant Human SIRT5 Protein

Recombinant Human SIRT5 Protein

Beta LifeScience SKU/CAT #: BL-4652PS
Catalog No.: BL-4652PS

Product Overview

Tag N/A
Host Species Human
Synonym Sirtuin 5, NAD-Dependent Lysine Demalonylase AndDesuccinylase Sirtuin-5,Mitochondria, NAD-Dependent Protein Deacylase Sirtuin-5Mitochondrial, Silent Mating Type Information Regulation 2 (S.Cerevisiae)Homolog 5, NAD-Dependent Deacetylase Sirtuin-5, Regulatory Protein SIR2 Homolog5, SIR2-Like Protein 5, SIR2L5, Sirtuin Type 5, Sir2-Like 5, EC 3.5.1.-.
Background SIRT5 belongs to the Sirtuin family of proteins, homologs to the yeast Sir2 protein. Sirtuin family members hold a sirtuin core domain and grouped into four classes. The protein encoded by this gene is included in class III of the sirtuin family. Human sirtuins functions were not yet determined; still, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies propose that human sirtuin could operate as intracellular regulatory protein with mono-ADP-ribosyltransferase activity.
Description SIRT5 Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain topological domain containing 300 aminoacids (34-310 a.a) and having a molecular weight of 32.5kDa. SIRT5 is fused to a 23amino acid His-tag at N-terminus and purified by proprietary chromatographictechniques.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKWQAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAGTKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHVVWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQGPCGTTLPEA LACHENETVS
Purity Greaterthan 90.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation SIRT5 protein solution (0.5mg/ml) containing 20mMTris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 30% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
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