Recombinant Human SEC61B Protein
Beta LifeScience
SKU/CAT #: BL-4182PS
Recombinant Human SEC61B Protein
Beta LifeScience
SKU/CAT #: BL-4182PS
Catalog No.: BL-4182PS
Product Overview
| Tag | N/A |
| Host Species | Human |
| Synonym | Protein transport protein Sec61 subunit beta, SEC61B, Sec61 Beta Subunit, Protein Translocation Complex Beta, Protein Transport Protein SEC61 Beta Subunit, Sec61 Complex Beta Subunit. |
| Background | The Sec61 complex is the key component of the protein translocation apparatus of the ER (endoplasmic reticulum) membrane. Oligomers of the Sec61 complex construct a transmembrane channel where proteins are translocated across and incorporated into the ER membrane. This complex consists of 3 membrane proteins- alpha, beta, and gamma. The SEC61B gene encodes the beta-subunit protein. The Sec61 subunits are also detected in the post-ER compartment, suggesting that these proteins can get out of the ER and recycle back. |
| Description | SEC61B Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 93a.a. (1-70 a.a) and having a molecular weight of 9.4kDa.SEC61B is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods. |
| Source | E.coli |
| AA Sequence | MGSSHHHHHH SSGLVPRGSH MGSMPGPTPS GTNVGSSGRS PSKAVAARAA GSTVRQRKNA SCGTRSAGRT TSAGTGGMWR FYTEDSPGLK VGP. |
| Purity | >85.0% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | SEC61B protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
