Recombinant Human S100A12 Protein

Name: Recombinant Human S100A12 Protein
Brand: Beta LifeScience
SKU: BLPSN-4112
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Accession	NP_005612.1
Synonym	CAAF1, CAGC, CGRP, ENRAGE, MRP-6, MRP6, p6, S100A12
Background	S1 proteinis a family of low molecular weight protein found in vertebrates characterized by twoEF-hand calcium-binding motifs. There are at least 21 different S1 proteins, and the name is derived from the fact that the protein is1%soluble in ammonium sulfateat neutralpH. Most S1 proteins are disulfide-linked homodimer, and is normally present in cells derived from theneural crest, chondrocytes, macrophages, dendritic cells, etc. S1 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S1-A12, also known as S1 calcium-binding protein A12, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, and S1A12, is a member of the S-11 family. Like the majority of S1 proteins, S1A12 is a dimer, with the interface between the two subunits being composed mostly of hydrophobic residues. The fold of S1A12 is similar to the other known crystal and solution structures of S1 proteins, except for the linker region between the two EF-hand motifs. S1A12 plays an important role in the inflammatory response.
Description	A DNA sequence encoding the native human S100A12 (NP_005612.1) (Met 1-Glu 92) was expressed.
Source	E.coli
Predicted N Terminal	Met 1
AA Sequence	Met 1-Glu 92
Molecular Weight	The recombinant human S100A12 consisting of 92 a.a. and has a calculated molecular mass of 10.6 kDa. It migrates as an 10 kDa band in SDS-PAGE under reducing conditions as predicted.
Purity	>98% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized recombinant human S100A12 at 2 ug/ml (100 ul/well) can bind human AGER with a linear range of 0.032-20 ug/ml.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.