Recombinant Human RUNX3 Protein

Name: Recombinant Human RUNX3 Protein
Brand: Beta LifeScience
SKU: BLA-7978P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Host Species	Human
Accession	Q13761
Synonym	Acute myeloid leukemia 2 protein Acute myeloid leukemia gene 2 AML 2 AML2 CBF alpha 3 CBF-alpha-3 CBFA 3 CBFA3 core binding factor Core binding factor alpha 3 subunit Core binding factor runt domain alpha subunit 3 Core binding factor subunit alpha 3 core-binding factor Core-binding factor subunit alpha-3 Oncogene AML 2 Oncogene AML-2 PEA2 alpha C PEA2-alpha C PEBP2 alpha C PEBP2-alpha C Pebp2a3 PEBP2aC Polyomavirus enhancer binding protein 2 alpha C subunit Polyomavirus enhancer-binding protein 2 alpha C subunit runt domain alpha subunit 3 runt related transcription factor 3 Runt-related transcription factor 3 RUNX 3 Runx3 RUNX3_HUMAN SL3 3 enhancer factor 1 alpha C subunit SL3-3 enhancer factor 1 alpha C subunit SL3/AKV core binding factor alpha C subunit SL3/AKV core-binding factor alpha C subunit Transcription factor AML2
Description	Recombinant Human RUNX3 Protein was expressed in E.coli. It is a Protein fragment
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MRSMVDVLAD HAGELVRTDS PNFLCSVLPS HWRCNKTLPV AFKVVALGDV PDGTVVTVMA GNDENYSAEL RNASAVMKNQ VARFNDLRFV GRSGRGKSFT LTITVFTNPT QVATYHRAIK VTVDGPREPR RHRQK
Molecular Weight	17 kDa including tags
Purity	>95% purity as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Liquid Solution
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.