Recombinant Human RTCA Protein

Beta LifeScience SKU/CAT #: BL-4161PS
Recombinant Human RTCA Protein

Recombinant Human RTCA Protein

Beta LifeScience SKU/CAT #: BL-4161PS
Catalog No.: BL-4161PS

Product Overview

Tag N/A
Host Species Human
Synonym RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1, RNA cyclase, RNA-3'-phosphate cyclase, RNA terminal phosphate cyclase domain-containing protein 1, RTC domain-containing protein 1, RTCA, RPC1.
Background RNA 3'-Terminal Phosphate Cyclase (RTCA) encodes a part of the RNA 3'-phosphate cyclase family. RTCA takes part in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. RTCA is involved in some phases of cellular RNA processing.
Description RTCA Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 389a.a. (1-366a.a) and having a molecular weight of 41.7kDa. RTCA is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL.
Purity >85.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation RTCA protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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