Recombinant Human RPS6KB1 Protein

Beta LifeScience SKU/CAT #: BL-4515PS
Recombinant Human RPS6KB1 Protein

Recombinant Human RPS6KB1 Protein

Beta LifeScience SKU/CAT #: BL-4515PS
Catalog No.: BL-4515PS

Product Overview

Tag His
Host Species Human
Synonym Ribosomal protein S6 kinase beta-1 isoform a, p70 S6KA, p70(S6K)-alpha, p70-alpha, p70-S6K, PS6K, S6K, S6K-beta-1, S6K1, STK14A, 70 kDa ribosomal protein S6 kinase 1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA.
Background Ribosomal Protein S6 Kinase Beta-1 Isoform A or RPS6KB1 is type of serine/threonine kinas. The protein effects on the PIP3 and phosphoinositide-dependent kinase-1 as part of the PI3 kinase pathway. Protein synthesis is induced by Phosphorylation of S6 in the ribosome. It was shown that phosphorylation of p70S6K at threonine 389 acts as mTOR activation indication and corresponded along with the inhibition of autophagy in different cases, nonetheless new studies has shown that the p70S6K activity has a constructive and increasing affect of autophagy.
Description RPS6KB1 expressed in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 533a.a. (1-525a.a.) and having a molecular weight of 60.2kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).RPS6KB1 is expressed with a 8a.a. His tag at C-Terminus and purified by unique purification methods.
Source Sf9
AA Sequence MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGELFMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEELLARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLLEHHH HHH.
Purity >85.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation RPS6KB1 protein solution (0.25mg/ml) contains 50mM Tris-HCl (pH 7.5), 30% glycerol, 0.5M NaCl, 2mM DTT and 0.1mM PMSF.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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