Recombinant Human RPL8 Protein

Beta LifeScience SKU/CAT #: BL-4529PS
Recombinant Human RPL8 Protein

Recombinant Human RPL8 Protein

Beta LifeScience SKU/CAT #: BL-4529PS
Catalog No.: BL-4529PS

Product Overview

Tag N/A
Host Species Human
Synonym 60S ribosomal protein L8, RPL8, L8.
Background 60S ribosomal protein L8 (RPL8) is a member of the ribosomal protein L2P family. Ribosomes consist of a small 40S subunit and a large 60S subunit. RPL8 is found in the cytoplasm and exists as a component of the 60S subunit where it is assumed to have role in aminoacyl-tRNA binding, specifically at the ribosomal subunit interface. In rats, the RPL8 protein connects with the 5.8S rRNA, and possibly participates in the binding of aminoacyl-tRNA, and is a component of the elongation factor 2-binding site at the ribosomal subunit interface.
Description RPL8 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 277a.a. (1-257 a.a) and having a molecular weight of 30.2kDa.RPL8 is fused to a 20a.a. His-tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK TVQEKEN.
Purity >90% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation RPL8 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 50% glycerol, 300mM NaCl and 2mM EDTA.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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