Recombinant Human RPL34 Protein

Name: Recombinant Human RPL34 Protein
Brand: Beta LifeScience
SKU: BL-4524PS
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Synonym	Ribosomal Protein L34, 60S Ribosomal Protein L34, Leukemia-Associated Protein, L34.
Background	Ribosomes, the organelles which catalyze protein synthesis, contain a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and nearly 80 structurally different proteins. RPL34 is a member of the L34E family of ribosomal proteins and it encodes a ribosomal protein which is a component of the 60S subunit. RPL34 is found in the cytoplasm and has been mapped to 4q though it was initially believed it is located at 17q21. Transcript variants which are a result of alternative splicing, alternative transcription initiation sites, and/or alternative polyadenylation exist; all encode the same protein. As is typical for genes encoding ribosomal proteins, there are several processed pseudogenes of this gene spread all over the genome.
Description	RPL34 Human Recombinant expressed in E.coli is a single, non-glycosylated polypeptide chain containing 140a.a. (1-117) and having a molecular weight of 15.7kDa.RPL34 is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSMVQRLTY RRRLSYNTAS NKTRLSRTPG NRIVYLYTKK VGKAPKSACG VCPGRLRGVR AVRPKVLMRL SKTKKHVSRA YGGSMCAKCV RDRIKRAFLI EEQKIVVKVL KAQAQSQKAK
Purity	>85.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	The RPL34 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.