Recombinant Human RNF114 Protein (denatured)

Name: Recombinant Human RNF114 Protein (denatured)
Brand: Beta LifeScience
SKU: BLA-7839P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Host Species	Human
Accession	Q9Y508
Synonym	1110008J21Rik AI225886 AW549494 MGC112560 OTTMUSP00000001082 OTTMUSP00000017392 PSORS12 RING finger protein 114 RN114_HUMAN RNF114 RP23-118A2.1 Zfp228 Zfp313 Zinc finger protein 228 Zinc finger protein 313 ZNF228 ZNF313
Description	Recombinant Human RNF114 Protein (denatured) was expressed in E.coli. It is a Full length protein
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSMAAQQRD CGGAAQLAGP AAEADPLGRF TCPVCLEVYE KPVQVPCGHV FCSACLQECL KPKKPVCGVCRSALAPGVRA VELERQIEST ETSCHGCRKN FFLSKIRSHV ATCSKYQNYI MEGVKATIKD ASLQPRNVPN RYTFPCPYCP EKNFDQEGLVEHCKLFHSTD TKSVVCPICA SMPWGDPNYR SANFREHIQR RHRFSYDTFV DYDVDEEDMM NQVLQRSIID Q
Molecular Weight	28 kDa including tags
Purity	>90% SDS-PAGE.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Liquid Solution
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Target Details

Target Function	E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates. In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity. Acts as negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3. May promote the ubiquitination of TRAF6 as well. Acts also as a negative regulator of T-cell activation. Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation. Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C. This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis.
Subcellular Location	Cytoplasm. Nucleus.
Database References	HGNC: 13094 OMIM: 612451 KEGG: hsa:55905 STRING: 9606.ENSP00000244061 UniGene: Hs.144949
Tissue Specificity	Expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. Highest levels in testis.

Gene Functions References

RNF114 is a new partner of A2O involved in the regulation of NF-kappaB activity that contributes to the control of signaling pathways modulating T cell-mediated immune response. PMID: 25165885
RNF114 over-expression had a promotion effect on T cell activation with an average 43.97% increment and the upregulatory roles showed a dose-dependent effect with 18.44% increment. PMID: 24631332
A single nucleotide plymorphism had an association with psoriasis in this Chinese Uygur population: SNP rs495337 in the gene encoding for zinc finger protein 313 (P < 0.001; OR = 0.80) PMID: 23617596
ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.). PMID: 23645206
Rare disease-associated variants in the promoter region of the RNF114 psoriasis susceptibility gene have been identified. PMID: 22205304
RNF114 regulates a positive feedback loop that enhances double-stranded RNA induced production of type I interferon. PMID: 21571784
ZNF313 is controlled at the transcriptional level, and a common mechanism controlling the basal transcription of ZNF313 gene exists. PMID: 20515446
ZNF313 is a novel psoriasis susceptibility gene. PMID: 18364390

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.