Recombinant Human RIOK3 Protein (Tagged)

Name: Recombinant Human RIOK3 Protein (Tagged)
Brand: Beta LifeScience
SKU: BLA-7812P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Host Species	Human
Accession	O14730
Synonym	Homolog of the Aspergillus nidulans sudD gene product RIO kinase 3 RIO kinase 3 (yeast) RIOK3 RIOK3_HUMAN Serine/threonine protein kinase RIO3 Serine/threonine-protein kinase RIO3 SUDD SudD (suppressor of bimD6, Aspergillus nidulans) homolog sudD homolog SudD suppressor of Aspergillus nidulans bimD6 homolog SudD suppressor of bimD6 homolog
Description	Recombinant Human RIOK3 Protein (Tagged) was expressed in Baculovirus infected Sf9 cells. It is a Full length protein
Source	Baculovirus infected Sf9 cells
AA Sequence	MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA VAEGPFITGE NIDTSSDLML AQMLQMEYDR EYDAQLRREE KKFNGDSKVS ISFENYRKVH PYEDSDSSED EVDWQDTRDD PYRPAKPVPT PKKGFIGKGK DITTKHDEVV CGRKNTARME NFAPEFQVGD GIGMDLKLSN HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL MYKMVNSGML ETITGCISTG KESVVFHAYG GSMEDEKEDS KVIPTECAIK VFKTTLNEFK NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLARMQ RAGIPCPTVV LLKKHILVMS FIGHDQVPAP KLKEVKLNSE EMKEAYYQTL HLMRQLYHEC TLVHADLSEY NMLWHAGKVW LIDVSQSVEP THPHGLEFLF RDCRNVSQFF QKGGVKEALS ERELFNAVSG LNITADNEAD FLAEIEALEK MNEDHVQKNG RKAASFLKDD GDPPLLYDE
Purity	>95% SDS-PAGE.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Liquid Solution
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

Target Details

Target Function	Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway. May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA.
Subcellular Location	Cytoplasm.
Protein Families	Protein kinase superfamily, RIO-type Ser/Thr kinase family
Database References	HGNC: 11451 OMIM: 603579 KEGG: hsa:8780 STRING: 9606.ENSP00000341874 UniGene: Hs.445511
Tissue Specificity	Widely expressed.

Gene Functions References

RIO kinase 3 (RIOK3) positively regulates the activity of the AKT/mTOR pathway in glioma cells. PMID: 29233656
the ectopic expression of RIOK3 or a phosphomimetic MDA5-S828D mutation attenuated MDA5-mediated signaling. PMID: 25865883
These findings demonstrate that RIOK3 is necessary for maintaining actin cytoskeletal organisation required for migration and invasion, biological processes that are necessary for hypoxia-driven metastasis. PMID: 25486436
Functionally, RIOK3 acts as a SUFU-dependent positive regulator of Hedgehog signaling. PMID: 24018050
Authors found that RIOK3 physically interacts with TBK1 and IRF3 and bridges the functions between TBK1 and IRF3 in the activation of type I interferon pathway. PMID: 24807708
RioK3 is a novel cytoplasmic component of pre-40S pre-ribosomal particle(s) in human cells, required for normal processing of the 21S pre-rRNA. PMID: 22418843
Like RIOK3, PAK4 promotes pancreas ductal cell motility and invasion in pancreatic cancer PMID: 19050074

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.