Recombinant Human RGS2 Protein

Name: Recombinant Human RGS2 Protein
Brand: Beta LifeScience
SKU: BLA-7774P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Host Species	Human
Accession	P41220
Synonym	Basic helix-loop-helix phosphoprotein G0S8 Cell growth inhibiting protein 31 Cell growth-inhibiting gene 31 protein G0 to G1 switch regulatory 8 24kD G0/G1 switch regulatory protein 8 G0S8 GOS8 OTTHUMP00000060765 Regulator of G protein signaling 2 Regulator of G protein signalling 2 24kD Regulator of G-protein signaling 2 Regulators of G protein signaling XRGSVI RGS 2 RGS2 RGS2_HUMAN
Description	Recombinant Human RGS2 Protein was expressed in E.coli. It is a Full length protein
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MQSAMFLAVQ HDCRPMDKSA GSGHKSEEKR EKMKRTLLKD WKTRLSYFLQ NSSTPGKPKT GKKSKQQAFI KPSPEEAQLW SEAFDELLAS KYGLAAFRAF LKSEFCEENI EFWLACEDFK KTKSPQKLSS KARKIYTDFI EKEAPKEINI DFQTKTLIAQ NIQEATSGCF TTAQKRVYSL MENNSYPRFL ESEFYQDLCK KPQITTEPHA T
Molecular Weight	27 kDa including tags
Purity	Greater than 90% SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Liquid Solution
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Target Details

Target Function	Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. It is involved in the negative regulation of the angiotensin-activated signaling pathway. Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle. Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein.
Subcellular Location	[Isoform 1]: Cell membrane. Cytoplasm. Nucleus, nucleolus.; [Isoform 2]: Cell membrane. Cytoplasm. Nucleus, nucleolus.; [Isoform 3]: Cell membrane. Cytoplasm. Nucleus, nucleolus.; [Isoform 4]: Cell membrane. Mitochondrion.
Database References	HGNC: 9998 OMIM: 600861 KEGG: hsa:5997 STRING: 9606.ENSP00000235382 UniGene: Hs.78944
Tissue Specificity	Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).

Gene Functions References

Results showed no association between genotypes and preeclampsia for polymorphisms rs5186, rs4606 in 3'UTR of genes ACVR2A, AGTR1 and RGS2 in women with preeclampsia PMID: 29593124
RGS2 is suggested as a novel AD biomarker (alongside other genes) toward early AD detection and future disease modifying therapeutics. PMID: 27701409
Upregulated RGS2 contributes significantly to the anti-fibrotic effects of pirfenidone in idiopathic pulmonary fibrosis. PMID: 27549302
study provides functional data for 16 human RGS2 missense variants on their effects on AT1R-mediated calcium mobilization and provides molecular understanding of those variants with functional loss in vitro. PMID: 28784619
Our results suggest that RGS2 might be involved in the pathogenesis of preeclampsia particularly in overweight women and contribute to their increased risk for hypertension and other types of cardiovascular disease later in life. PMID: 27558088
Data suggest that epigenetic changes in histone acetylation and DNA methylation may contribute to the repression of RGS2 (regulator of G-protein signaling 2) expression in chemo-resistant ovarian cancer cells; regulation of HDAC1 (histone deacetylase 1) and DNMT1 (DNA methyltransferase 1) contribute to the suppression of RGS2. PMID: 28102109
The RGS2 (-391, C>G) genetic polymorphism may serve as a biomarker to predict a patient's response to antihypertensive drug therapy PMID: 25849301
MIR4717 regulates human RGS2 and contributes to the genetic risk towards anxiety-related traits. PMID: 25847876
FBXO44-mediated degradation of RGS2 protein uniquely depends on a Cul4B/DDB1 complex. PMID: 25970626
RGS2 polymorphisms were found to be associated with anxiety disorders and dimensional as well as intermediate phenotypes of anxiety. PMID: 25740197
RGS2 localizes to the mitotic spindle in a Nek7-dependent manner, and along with Nek7 contributes to spindle morphology and mitotic spindle pole integrity. PMID: 25664600
genetic variations and increased inflammatory cytokines can lead to RGS2 repression, which exacerbates AHR and airway remodeling in asthma. PMID: 25368964
Data show that regulator of G protein signaling 2 (RGS2) was stabilized by deubiquitinase monocyte chemotactic protein-induced protein 1 (MCPIP1). PMID: 25187114
Epigenetic repression of RGS2 by UHRF1 contributes to bladder cancer progression. UHRF1 inhibits RGS2 expression by increasing the methylation of CpG nucleotides of the RGS2 promoter. PMID: 25323766
ACE and RGS2 genotypes are not associated with the development of hypertension in patients with type 1 diabetes mellitus. PMID: 24562335
This work highlights the role of RGS2 as a key regulator of LRRK2 activity, function and neuronal toxicity. PMID: 24794857
study found that wild-type Met-Gln-Rgs2 and its mutant, Met-Arg-Rgs2, were destroyed by the Ac/N-end rule pathway, which recognizes N(alpha)-terminally acetylated (Nt-acetylated) proteins PMID: 25766235
The results of this study are consistent previous results and support the hypothesis that polymorphic loci RGS2 gene associated with risk of extrapyramidal symptoms induced by typicalneuroleptics-haloperidol, and are involved in schizophrenia pathway. PMID: 25509855
Women carrying the rs4606 CG or GG genotype are at elevated risk for developing hypertension after delivery following a preeclamptic pregnancy. PMID: 24593135
This meta-analysis revealed that the RGS2 1891-1892del TC polymorphism and CYP4A11 T8590C polymorphism were associated with hypertension risk. PMID: 23859711
observations support the concept of a functional activation-dependent p63RhoGEF-Galphaq-RGS2 complex PMID: 24299002
ectopic expression of R4 subfamily members RGS2, RGS3, RGS4, and RGS5 reduced activated PAR1 wild-type signaling, whereas signaling by the PAR1 AKKAA mutant was minimally affected. PMID: 24297163
RGS2 promoter of hypertensive patients abolished HSF1-regulated expression of RGS2, suggesting that activated HSF1 is involved in blood pressure regulation via modulation of RGS2 expression PMID: 23587726
The study was unable to replicate or extend prior association findings between RGS2 variants and various anxiety-related phenotypes using a large, independent sample. PMID: 23277133
Data show that miR-22 specifically interacts with the 3' UTRs of the Rcor1, Rgs2 and HDAC4 mRNAs. PMID: 23349832
It was shown that the RGS2 rs4606 single nucleotide polymorphism may affect the risk and progression of preeclampsia. PMID: 23339167
The variants and their frequencies in RGS2 gene in Kazakh hypertensives may have ethnic differences when compared with other populations. PMID: 21529451
In vivo treatment with digoxin led to increased RGS2 protein levels in heart and kidney. PMID: 22695717
Loss of RGS2 also increased ASM mass and stimulated airway smooth muscle cell growth via extracellular signal-regulated kinase and phosphatidylinositol 3-kinase pathways PMID: 22704538
Reduced RGS2 protein expression in human prostate cancer specimens. PMID: 21500190
Data identify RGS2 gene expression as a genomic mechanism of bronchoprotection that is induced by glucocorticoids plus beta(2)-adrenoceptor agonist (LABA) in airway smooth muscle. PMID: 22080612
Identification of a cAMP-response element in the regulator of G-protein signaling-2 (RGS2) promoter as a key cis-regulatory element for RGS2 PMID: 22057271
Findings both identify RGS2 downregulation as a novel compensatory response in HD neurons and suggest that RGS2 inhibition might be considered as an innovative target for neuroprotective drug development. PMID: 21779398
genetic polymorphisms in RGS2 are associated with intima-media thickening of carotid artery in humans. PMID: 21451528
The haplotypes T-G-C and T-C-T showed significant association and protective effect on Panic disorder; results provide support for an association of RGS2 with panic disorder in a Japanese population PMID: 21438143
The essential roles of both N-terminal subdomains for the potent inhibitory activity of RGS2 on AT1 receptor signaling. PMID: 21291998
down-regulation of RGS2 might play an important role in colorectal cancer (CRC) metastasis and predict poor prognosis in stage II and III CRC patients. PMID: 20001967
These findings suggest that RGS2 may not be genetically involved in the biological susceptibility to panic disorder in Japanese. PMID: 20847599
platelet Gs signaling defect caused by a heterozygous RGS2 variant that results in a unique mutational mechanism, such as the differential use of translation initiation sites resulting in different functional RGS2 isoforms. PMID: 20403096
The D allele of 1891-1892 TC insertion/deletion of RGS2 might be an independent risk factor for hypertension in Xinjiang Kazakhs. PMID: 20662725
data suggest that RGS2 and RGS8 differentially associate with MCHR1 and may represent two distinct modes of signaling mechanisms in vivo PMID: 20633139
Reduced RGS2 expression contributes to resistance to antihypertensive agents through poor negative feedback on the effects of aldosterone and of other vasoactive agents. PMID: 20375904
The D allele of the 1891-1892TC insertion/deletion locus of the RGS2 gene is an independent risk factor for hypertension in a Chinese population. PMID: 20140863
Ischemic stress increases RGS2 expression and that this condition contributes to enhanced apoptosis in C6 cells and primary astrocytes. PMID: 20032508
Phosphoglycerate kinase 2 (PGK2) showed a difference between follicular cells from follicles leading to a pregnancy or developmental failure. PMID: 19778949
rs4606 does not affect AIEPSs in Japanese subjects. PMID: 19931593
RGS2 plays a role in regulating purinergic signaling in human broncheotracheal cells PMID: 12356577
Identification of RGS2 and type V adenylyl cyclase interaction sites. PMID: 12604604
RGS-2 level in human clinical condition characterized by altered vascular tone. Importance of RGS-2 as key regulator element for Ang II signaling. Links between Bartter's/Gitelman's syndrome genetic abnormalities and abnormal vascular tone regulation. PMID: 15292363
TSH-dependent RGS 2 mRNA expression and the suppression of TSH-G(q)alpha signaling by the overexpression of RGS 2 imply that RGS 2 is involved in TSHR-induced G(q) signal transduction. PMID: 15362969

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.