Recombinant Human RARA Protein

Beta LifeScience SKU/CAT #: BL-4136PS
Recombinant Human RARA Protein

Recombinant Human RARA Protein

Beta LifeScience SKU/CAT #: BL-4136PS
Catalog No.: BL-4136PS

Product Overview

Tag His
Host Species Human
Synonym Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1,RAR, NR1B1, RARA.
Background Retinoic acid receptor alpha (RAR) belongs to the large family of ligand responsive gene regulatory proteins that includes receptors for steroid and thyroid 60. These proteins contain two highly conserved domains that are involved in determining their DNA and ligand-binding activities. There are three isotypes of RAR proteins: alpha, beta, and gamma. The RAR proteins are encoded by distinct genetic loci and possess distinct transcriptional properties. Typically, RAR-alpha represses target gene transcription in the absence of hormone, whereas RAR-beta and gamma fail to repress under these conditions. RARA is a receptor for retinoic acid that has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. RARA controls cell function by directly regulating gene expression.Chromosomal aberrations involving RARA cause acute promyelocytic leukemia (APL).
Description RARA Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 127a.a. (68-173 a.a.) and having a molecular weight of 14kDa (molecular weight on SDS-PAGE will appear higher). The RARA fused to a 21a.a. his tag at N-terminus and is purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MSEEIVPSPP SPPPLPRIYK PCFVCQDKSS GYHYGVSACE GCKGFFRRSI QKNMVYTCHR DKNCIINKVT RNRCQYCRLQ KCFEVGMSKESVRNDRNKKK KEVPKPE.
Purity >95.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The protein solution (1mg/1ml) contains 20mM Tris-HCl pH-7.5, 0.1M NaCl & 5mM b-mercaptoethanol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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