Recombinant Human RAP1A Protein

Beta LifeScience SKU/CAT #: BL-3496PS
Recombinant Human RAP1A Protein

Recombinant Human RAP1A Protein

Beta LifeScience SKU/CAT #: BL-3496PS
Catalog No.: BL-3496PS

Product Overview

Tag N/A
Host Species Human
Synonym KREV1, G-22K, C21KG, RAP1A, RAP1, Ras-related protein Rap-1A, GTP-binding protein smg p21A, Ras-related protein Krev-1.
Background RAP1A is part of the family of RAS-related proteins which possess 50% amino acid identity with the classical RAS proteins and have various structural characteristicsin common. RAP proteins differ from RAS proteins in their 61st amino acid, glutamine in RAS is replaced by threonine in RAP proteins. RAP1A induces morphological reversion of a cell line transformed by a Ras oncogene and counteracts the mitogenic function of Ras, since it can interact with Ras GAPs and RAF in a competitive manner.
Description RAP1A Recombinant E.coli expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 201a.a. (1-181 a.a.) and having a molecular weight of 22.8 kDa. The RAP1A is fused to 20a.a. His-Tag at N-terminus and purified by conventional chromatography.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C.
Purity >85.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation 0.25mg/ml solution containing 20mM Tris HCL pH-8.0, 5mM DTT, 0.2M NaCl & 40% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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