Recombinant Human RANBP1 Protein
Beta LifeScience
SKU/CAT #: BL-4457PS
Recombinant Human RANBP1 Protein
Beta LifeScience
SKU/CAT #: BL-4457PS
Catalog No.: BL-4457PS
Product Overview
| Tag | N/A |
| Host Species | Human |
| Synonym | RAN binding protein 1, HTF9A, ran-specific GTPase-activating protein, RanBP1. |
| Background | RANBP1 is a member of a family of proteins which bind Ran GTPase. Ran GTPase is vital for cell cycle control, mRNA processing, nuclear transport, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. RANBP1 does not stimulate GTPase activity of RAN however RANBP1 does distinctly raise GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). Additionally, RANBP1 operates as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. |
| Description | RANBP1 Human Recombinant expressed inE. coli is a single polypeptide chain containing 225a.a. (1-201) and having a molecular weight of 25.8kDa (molecular size on SDS-PAGE will appear higher).RANBP1 is fused to a 24a.a. His-tag at N-terminus and purified by unique purification methods. |
| Source | E.coli |
| AA Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ. |
| Purity | >95% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | The RANBP1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
