Recombinant Human RAB35 Protein

Beta LifeScience SKU/CAT #: BL-3480PS
Recombinant Human RAB35 Protein

Recombinant Human RAB35 Protein

Beta LifeScience SKU/CAT #: BL-3480PS
Catalog No.: BL-3480PS

Product Overview

Tag N/A
Host Species Human
Synonym Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAB35, RAB1C, RAY.
Background Ras-related protein Rab-35 (RAB35) is restricted to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis, which is consistent with a central requirement for Rab35-regulated recycling during cell division.
Description RAB35 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 221a.a. (1-201 a.a.) and having a molecular weight of 25.2kDa.RAB35 is fused to a 20a.a. His-tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C.
Purity >90% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation RAB35 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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