Recombinant Human RAB35 Protein
Beta LifeScience
SKU/CAT #: BL-3480PS
Recombinant Human RAB35 Protein
Beta LifeScience
SKU/CAT #: BL-3480PS
Catalog No.: BL-3480PS
Product Overview
| Tag | N/A |
| Host Species | Human |
| Synonym | Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAB35, RAB1C, RAY. |
| Background | Ras-related protein Rab-35 (RAB35) is restricted to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis, which is consistent with a central requirement for Rab35-regulated recycling during cell division. |
| Description | RAB35 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 221a.a. (1-201 a.a.) and having a molecular weight of 25.2kDa.RAB35 is fused to a 20a.a. His-tag at N-terminus and purified by unique purification methods. |
| Source | E.coli |
| AA Sequence | MGSSHHHHHH SSGLVPRGSH MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C. |
| Purity | >90% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | RAB35 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
