Recombinant Human QKI Protein

Beta LifeScience SKU/CAT #: BL-4129PS

Recombinant Human QKI Protein

Beta LifeScience SKU/CAT #: BL-4129PS
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag N/A
Host Species Human
Synonym Hqk, hqkI, QK, QK1, QK3, Protein quaking, QuakingHomolog, KH Domain RNA Binding, RNA Binding Protein HQK, HKQ, KHDomain Containing, RNA Binding.
Background KH Domain RNA Binding also known as QKI is aRNA-binding protein which regulates pre-mRNA splicing, export of mRNAs from thenucleus, protein translation, and mRNA stability. QKIis involved in myelinizationand oligodendrocyte differentiation, and it may play a role in schizophrenia.Defects or deletions in the QKIare linked with astrocytic tumors and may be implicated in the pathogenesis ofschizophrenia. Among the diseases associated with QKI are6q terminal deletion syndrome, and schizophrenia.
Description QKI Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 364a.a. (1-341 a.a) and having a molecular weight of 40.1kDa.QKI is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMVGEMET KEKPKPTPDY LMQLMNDKKL MSSLPNFCGI FNHLERLLDE EISRVRKDMY NDTLNGSTEK RSAELPDAVG PIVQLQEKLY VPVKEYPDFN FVGRILGPRG LTAKQLEAET GCKIMVRGKG SMRDKKKEEQ NRGKPNWEHL NEDLHVLITV EDAQNRAEIK LKRAVEEVKK LLVPAAEGED SLKKMQLMEL AILNGTYRDA NIKSPALAFS LAATAQAAPR IITGPAPVLP PAALRTPTPA GPTIMPLIRQ IQTAVMPNGT PHPTAAIVPP GPEAGLIYTP YEYPYTLAPA TSILEYPIEP SGVLGAVATK VRRHDMRVHP YQRIVTADRA ATGN.
Purity >85.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation QKI protein solution (0.25mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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