Recombinant Human PURB Protein

Beta LifeScience SKU/CAT #: BL-4127PS
Recombinant Human PURB Protein

Recombinant Human PURB Protein

Beta LifeScience SKU/CAT #: BL-4127PS
Catalog No.: BL-4127PS

Product Overview

Tag N/A
Host Species Human
Synonym Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B, PURB, PURBETA.
Background Purine-Rich Element Binding Protein B (PURB) is a single-stranded DNA-binding protein which takes part in the dendritic transport of a subset of mRNAs. PURB operates as repressor in myoblasts and fibroblasts in the control of vascular smooth muscle alpha-actin gene transcription. PURB binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in various eukaryotes from yeasts through humans.
Description PURB Human Recombinant expressed in E.coli is a single, non-glycosylated polypeptide chain containing 335a.a. (1-312) and having a molecular weight of 35.6 kDa.PURB is fused to a 23a.a. His-Tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMADGDSG SERGGGGGPC GFQPASRGGG EQETQELASK RLDIQNKRFY LDVKQNAKGR FLKIAEVGAG GSKSRLTLSM AVAAEFRDSL GDFIEHYAQL GPSSPEQLAA GAEEGGGPRR ALKSEFLVRE NRKYYLDLKE NQRGRFLRIR QTVNRGGGGF GAGPGPGGLQ SGQTIALPAQ GLIEFRDALA KLIDDYGGED DELAGGPGGG AGGPGGGLYG ELPEGTSITV DSKRFFFDVG CNKYGVFLRV SEVKPSYRNA ITVPFKAWGK FGGAFCRYAD EMKEIQERQR DKLYERRGGG SGGGEESEGE EVDED.
Purity >95% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The PURB solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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