Recombinant Human PITPnm 3 Protein

Name: Recombinant Human PITPnm 3 Protein
Brand: Beta LifeScience
SKU: BLA-7047P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Host Species	Human
Accession	Q9BZ71
Synonym	CORD5 Membrane associated phosphatidylinositol transfer protein 3 membrane-associated 3 Membrane-associated phosphatidylinositol transfer protein 3 MGC157740 MGC157741 NIR 1 NIR-1 NIR1 Phosphatidylinositol transfer protein Phosphatidylinositol transfer protein, membrane-associated 3 PITM3_HUMAN PITPNM PITPnm 3 PITPNM family member 3 Pitpnm3 PYK2 N terminal domain interacting receptor 1 Pyk2 N-terminal domain-interacting receptor 1 RDGBA3 Retinal degeneration B alpha 3
Description	Recombinant Human PITPnm 3 Protein was expressed in E.coli. It is a Protein fragment
Source	E.coli
Molecular Weight	26 kDa
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Lyophilised
Stability	The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution	See related COA
Unit Definition	For Research Use Only
Storage Buffer	Shipped at 4°C. Store at -20°C.

Target Details

Target Function	Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes (in vitro). Binds calcium ions.
Subcellular Location	Endomembrane system; Peripheral membrane protein.
Protein Families	PtdIns transfer protein family, PI transfer class IIA subfamily
Database References	HGNC: 21043 OMIM: 600977 KEGG: hsa:83394 STRING: 9606.ENSP00000262483 UniGene: Hs.183983
Associated Diseases	Cone-rod dystrophy 5 (CORD5)
Tissue Specificity	Detected in brain and spleen, and at low levels in ovary.

Gene Functions References

CCL18 can increase the invasive ability of non-small cell lung cancer cells by binding to its receptor Nir1. PMID: 26756176
CCL18 enhances hepatocellular carcinoma (HCC) cell migration, invasion, and epithelial-mesenchymal transition (EMT) through the expression of PITPNM3 and the activation of the NF-kappaB signaling pathway. PMID: 26449829
Data indicate that PYK2 N-terminal domain interacting receptor 1 (Nir1) could induce epithelial-mesenchymal transition by stabilising Snail via the PI3K/Akt/GSK3beta/Snail signalling pathway through binding to CCL18. PMID: 24001613
Our observations of the PITPNM3 p.Q626H mutation carriers confirm that CORD5 is a disease not to mix with other retinal degenerations mapped to 17p13. PMID: 22405330
CCL18 derived from Tumor-associated macrophages (TAMs) lays a critical role in promoting breast cancer metastasis via its receptor, PITPNM3. PMID: 21481794
Thus, mutations in PITPNM3 do not appear to be a major cause of cone or cone-rod dystrophy. PMID: 20590364
Our finding on the first mutation in the human homologue of Drosophila rdgB indicates novel pathways and a potential important role of the PITPNM3 in mammalian phototransduction. PMID: 17377520
Mutation in the PYK2-binding domain of PITPNM3 causes autosomal dominant cone dystrophy (CORD5) in two Swedish families. PMID: 18188949

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.