Recombinant Human PHF5A Protein

Beta LifeScience SKU/CAT #: BLA-6937P

Recombinant Human PHF5A Protein

Beta LifeScience SKU/CAT #: BLA-6937P
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Product Overview

Host Species Human
Accession Q7RTV0
Synonym 1110007B08Rik Ac2 246 bK223H9.2 INI MGC1346 OTTHUMP00000198827 PHD finger 5a PHD finger like domain containing protein 5A PHD finger like domain protein 5A PHD finger protein 5A PHD finger-like domain protein 5A PHD finger-like domain-containing protein 5A Phf5a PHF5A_HUMAN Rds3 SAP14b SF3b14b Splicing factor 3B associated 14 kDa protein Splicing factor 3B-associated 14 kDa protein Transcription factor INI
Description Recombinant Human PHF5A Protein was expressed in E.coli. It is a Full length protein
Source E.coli
AA Sequence MGSSHHHHHHSSGLVPRGSHMGSMAKHHPDLIFCRKQAGVAIGRLCEKCD GKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTI QEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR
Molecular Weight 15 kDa including tags
Purity Greater than 85% SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Liquid Solution
Stability The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution See related COA
Unit Definition For Research Use Only
Storage Buffer Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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