Recombinant Human PHF5A Protein

Beta LifeScience SKU/CAT #: BL-4098PS
Recombinant Human PHF5A Protein

Recombinant Human PHF5A Protein

Beta LifeScience SKU/CAT #: BL-4098PS
Catalog No.: BL-4098PS

Product Overview

Tag N/A
Host Species Human
Synonym PHD finger protein 5A, bK223H9.2, INI, Rds3, SAP14b, SF3b14b, PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, PHF5A.
Background PHD Finger Protein 5A, also known as PHF5A, is a part of the PHD-finger superfamily and acts as a chromatin-associated protein. PHF5A is a subunit of the splicing factor 3b protein complex which along with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent way and anchors the U2 snRNP to the pre-mRNA. PHF5A holds a PHD-finger-like domain which is flanked by highly basic N- and C-termini.
Description PHF5A Human Recombinant expressed in E. coli is a single polypeptide chain containing 133a.a. (1-110) and having a molecular weight of 14.8kDa. PHF5A is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMAKHHPD LIFCRKQAGV AIGRLCEKCD GKCVICDSYV RPCTLVRICD ECNYGSYQGR CVICGGPGVS DAYYCKECTI QEKDRDGCPK IVNLGSSKTD LFYERKKYGF KKR.
Purity >85% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The PHF5A solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Recently viewed