Recombinant Human PDAP1 Protein

Beta LifeScience SKU/CAT #: BL-4087PS
Recombinant Human PDAP1 Protein

Recombinant Human PDAP1 Protein

Beta LifeScience SKU/CAT #: BL-4087PS
Catalog No.: BL-4087PS

Product Overview

Tag N/A
Host Species Human
Synonym PAP1, HASPP28.
Background PDAP1, increases PDGFA-stimulated cell growth in fibroblasts, however inhibits the mitogenic effect of PDGFB. PDAP1 expression is induced by TNF-alpha, and cells overexpressing PDAP1 show significantly less apoptosis on exposure to TNF-alpha.
Description PDAP1 Recombinant Human expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 189a.a. (1-181 a.a.) and having a molecular weight of 21.7 kDa. The PDAP1 is fused to an 8a.a. His-Tag at C-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MPKGGRKGGH KGRARQYTSP EEIDAQLQAE KQKAREEEEQ KEGGDGAAGD PKKEKKSLDS DESEDEEDDY QQKRKGVEGL IDIENPNRVA QTTKKVTQLD LDGPKELSRR EREEIEKQKA KERYMKMHLA GKTEQAKADL ARLAIIRKQR EEAARKKEEE RKAKDDATLS GKRMQSLSLN KLEHHHHHH.
Purity >90.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The PDAP1 solution (1mg/ml) contains 20mM Tris-HCl pH-8, 2mM DTT, 0.1M NaCl, 0.1mM PMSF & 20% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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