Recombinant Human PARD6B Protein
Beta LifeScience
SKU/CAT #: BL-4077PS
Recombinant Human PARD6B Protein
Beta LifeScience
SKU/CAT #: BL-4077PS
Catalog No.: BL-4077PS
Product Overview
| Tag | N/A |
| Host Species | Human |
| Synonym | Partitioning defective 6 homolog beta, PAR-6 beta, PAR-6B, PARD6B, PAR6B. |
| Background | Partitioning defective 6 homolog beta (PARD6B) belongs to the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. Cellular asymmetry is crucial for the development of multicellular organisms. PARD (partitioning-defective) proteins have central roles in asymmetric cell division and polarized growth, whereas Cdc42 and Rac mediate establishment of cell growth and polarity and contribute to oncogenic transformation by Ras. PARD6B is expressed in pancreas and in both the adult and the fetal kidney, and is weakly expressed in the placenta and the lung. |
| Description | PARD6B Human Recombinant expressed in E.coli is a single, non-glycosylated polypeptide chain containing 395a.a. (1-372) and having a molecular weight of 43.6kDa.PARD6B is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods. |
| Source | E.coli |
| AA Sequence | MGSSHHHHHH SSGLVPRGSH MGSMNRSHRH GAGSGCLGTM EVKSKFGAEF RRFSLERSKP GKFEEFYGLL QHVHKIPNVD VLVGYADIHG DLLPINNDDN YHKAVSTANP LLRIFIQKKE EADYSAFGTD TLIKKKNVLT NVLRPDNHRK KPHIVISMPQ DFRPVSSIID VDILPETHRR VRLYKYGTEK PLGFYIRDGS SVRVTPHGLE KVPGIFISRL VPGGLAQSTG LLAVNDEVLE VNGIEVSGKS LDQVTDMMIA NSRNLIITVR PANQRNNVVR NSRTSGSSGQ STDNSLLGYP QQIEPSFEPE DEDSEEDDII IEDNGVPQQI PKAVPNTESL ESLTQIELSF ESGQNGFIPS NEVSLAAIAS SSNTEFETHA PDQKLLEEDG TIITL. |
| Purity | >85.0% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | The PARD6B solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea, 20% glycerol and 0.2M NaCl. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
